Translational_Unit

Part:BBa_K4310016:Design

Designed by: Seungchan Oh   Group: iGEM22_UIncheon   (2022-10-11)


Heat shock inducible promoter + phiC31 recombinase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 842
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2854
    Illegal NheI site found at 3024
    Illegal NheI site found at 4066
    Illegal NheI site found at 6778
    Illegal PstI site found at 842
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 226
    Illegal BglII site found at 1211
    Illegal BglII site found at 2512
    Illegal BamHI site found at 3249
    Illegal BamHI site found at 3279
    Illegal BamHI site found at 3365
    Illegal BamHI site found at 3375
    Illegal XhoI site found at 3206
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 842
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 842
    Illegal NgoMIV site found at 2228
    Illegal NgoMIV site found at 2446
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1393
    Illegal BsaI site found at 4447
    Illegal BsaI site found at 7709
    Illegal BsaI.rc site found at 1672
    Illegal BsaI.rc site found at 4726
    Illegal BsaI.rc site found at 5438
    Illegal BsaI.rc site found at 7988
    Illegal SapI site found at 1857
    Illegal SapI site found at 2387
    Illegal SapI site found at 3736
    Illegal SapI site found at 4965
    Illegal SapI.rc site found at 2372
    Illegal SapI.rc site found at 2421
    Illegal SapI.rc site found at 4061
    Illegal SapI.rc site found at 4999


Design Notes

To make compatible to our assembly, CAMV 35S, mCherry and Tnos are amplified by overhang primers.


Source

CAMV 35S: BBa_J428074 from distribution 2022 attB:BBa_K2460010 attP:BBa_K2460011 phiC31: BBa_K1742004 mCherry: BBa_J428079 from distribution 2022 Tnos: BBa_J428082 from distribution 2022 pCvHSP70A: BBa_K4310005 eGFP: BBa_K431006 Kozak: BBa_K4310003

References